Cambridge Healthtech Institute’s 14th Annual

Difficult-to-Express Proteins

Overcoming Expression Challenges

April 8-9, 2019

 

Proteins are each unique and bring unique challenges when attempting to tame them into submission. CHI’s 14th annual Difficult-to-Express Proteins conference examines the challenges researchers encounter when striving for high-yield production of “difficult-to-express” proteins (DTEPs) and the strategies and technologies that have proven successful in overcoming those challenges. Some of the difficulties encountered include solubility, proper folding, inability to crystallize and aggregation and formation of inclusion bodies. Researchers employ a range of problem-specific solutions to achieve expression including genetic modifications, manipulating how a target protein is produced and employing protein tags. In addition, the identification of DNA coding sequences along with the use of high-throughput approaches has brought about significant improvements. The Difficult-to-Express Proteins conference provides the latest developments in improving yield for DTEPs through Case Studies and breakthrough data.

Preliminary Agenda

INNOVATING PROCESSES FOR DTEPs

KEYNOTE PRESENTATION:
A High-Throughput Platform to Express All Human Cell Surface Proteins

James Love, PhD, COO, Institute for Protein Innovation

Modulation of STEAP2 Conformation by the Cholesterol Content of Cellular Membrane: An In-Depth Study of Conformational Epitope Located in the Second Extracellular Loop

Haruki Hasegawa, PhD, Principal Scientist, Biologics – Protein Technology, Amgen, Inc.

9g/l in 90 hours: Development of C1 into a Next-Generation Therapeutic Protein Production System

Ronen Tchelet, Vice President, R&D, Dyadic International, Inc.


EXPRESSING ANTIBODIES

Utilizing a Regulated Targeted Integration (RTI) Cell Line Development (CLD) Approach to Systematically Investigate What Makes an Antibody Difficult to Express

Shahram Misaghi, PhD, Senior Scientist, Early Stage Cell Culture, Genentech, Inc.

Efficient and Inexpensive Transient Expression of Multispecific Multivalent Antibodies in Expi293 Cells

Greta Hultqvist, PhD, Associate Senior Lecturer, Pharmaceutical Biosciences, Protein Drug Design, Uppsala University

NEXT-GEN TOOLS & STRATEGIES FOR DTEPs

FEATURED PRESENTATION:
MicroED: Cryo-Electron Diffraction of 3D Microcrystals

Brent Nannenga, PhD, Assistant Professor, Chemical Engineering, Arizona State University

Development of a High-Yielding Expression Platform for the Introduction of Non-Natural Amino Acids

Gargi Roy, MSc, Scientist, Antibody Discovery and Protein Engineering, MedImmune, LLC

Presentation to be Announced


CHO-BASED EXPRESSION FOR DTEPs

Teaching an Old Dog New Tricks: Making CHO Cell Line Development Ready for the Difficult-to-Express Protein Challenge

Simon Fischer, PhD, Head, Cell Line Development, Global Bioprocess & Pharmaceutical Development, Boehringer Ingelheim Pharma GmbH & Co. KG

Luncheon Presentation to be Announced


MEMBRANE PROTEINS

Kinetics of Membrane Protein-Detergent Interactions Depend on Protein Electrostatics

Liviu Movileanu, PhD, Professor, Physics, Syracuse University

Production of Human Integral Membrane Proteins Using Baculovirus and BacMam

Nicola Burgess-Brown, PhD, Principal Investigator, Biotechnology, Structural Genomics Consortium, University of Oxford

INSECT CELLS & THE BACULOVIRUS EXPRESSION VECTOR SYSTEM

A New Single-Step Protocol for Rapid Baculovirus-Driven Protein Production in Insect Cells

Sabine Suppmann, PhD, Head, Recombinant Protein Production, Max Planck Institute for Biochemistry

Tools for Studying the RAS/RAF/MEK Pathway: Using the BEVS to Produce Complexes and Native Proteins

William Gillette, PhD, Principal Scientist, RAS Reagents Core, and Deputy Director, Protein Expression Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research (FNL)


* The program is subject to change without notice, due to unforeseen reason.

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