Cambridge Healthtech Institute’s 5th Annual

Protein Expression System Engineering

Gene to Cell Line

April 11-12, 2019


CHI’s 5th annual Protein Expression System Engineering conference examines the functioning of the cellular machinery harnessed during protein biosynthesis and how to engineer hosts to efficiently express a protein of interest. The intricate steps required to achieve properly folded protein will be discussed, including verification and sequence analysis of the gene, codon optimization, vector construction, selecting and optimizing a clone and selecting a host system. In addition, engineering host cells to sustain expression for longer time periods will be discussed, along with overcoming cellular stress response to produce and secrete functionally active recombinant proteins.

Preliminary Agenda


Mammalian Synthetic Biology: Foundations and Application to Cell Line Engineering

Ron Weiss, PhD, Professor, Biological Engineering, Massachusetts Institute of Technology (MIT)

Cell-Free Synthetic Biology for Therapeutics, Sensing, and Remediation

David Karig, PhD, Associate Professor, Systems and Synthetic Biology, Bioengineering, Clemson University

Steering N-Glycosylation of Recombinant Proteins Using Systems Engineering

Michael J. Smanski, PhD, Assistant Professor, Biochemistry, Molecular Biology & Biophysics, Biotechnology Institute, University of Minnesota


A Multi-Landing Pad DNA Integration Platform for Mammalian Cell Engineering

Liliana Wroblewska, PhD, Principal Scientist, Biomedicine Design, Pfizer, Inc.

Improving Cytidine and Adenine Base Editors by Expression Optimization and Ancestral Reconstruction

Luke Koblan, PhD, Scientist, Chemical Biology, Chemistry & Chemical Biology, Harvard University

Engineering CHO Metabolic Essential Gene for Efficient and High Expressing Clones Suited for Perfusion Processes

Hatim Motiwala, PhD, Head, Cell Line Engineering and Bio-Analytical Sciences, Enzene Biosciences Limited


Methylation Analysis of Cell Lines with Varying Productivities

Susan Sharfstein, PhD, Professor, Nanobioscience, Nanoscale Science and Engineering, SUNY Polytechnic Institute

Production of Antimicrobial Peptides Using Protein-Cage Carrier Proteins

Mimi Cho Yung, PhD, Biomedical Scientist, Lawrence Livermore National Laboratory (LLNL)


Molecular Approaches that Improve Soluble Protein Yields from Bacterial Expression Systems

Christopher H. Gray, PhD, Staff Scientist & Team Leader (Structural Biology), Drug Discovery Program, CRUK Beatson Institute

Recombinant (Membrane) Protein Production in Yeast

Roslyn M. Bill, DPhil, Professor, Biotechnology, and Associate Dean, Research, Aston University

Development of the Filamentous Fungus Myceliophthora thermophila C1 into a Next-Generation Therapeutic Protein Production System

Markku Saloheimo, PhD, Senior Principal Scientist, Industrial Biotechnology and Food Solutions, VTT Technical Research Centre of Finland, Ltd.

* The program is subject to change without notice, due to unforeseen reason.

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