antibody engineering and design



The 4th Annual Engineering Antibodies conference at PEGS Europe presents the latest antibodies to watch, and explores technologies and new approaches to discover, design and engineer novel modalities and new platforms for existing and challenging targets. The conference will also explore new techniques such as deep sequencing, in silico engineering and gene editing to help further the understanding of the sequence and structure-function of the molecules, and close the gap from bench to clinic.

Final Agenda

WEDNESDAY 20 NOVEMBER

07:45 Registration and Morning Coffee

NEW MODALITIES AND PLATFORMS

08:30 Chairperson’s Opening Remarks

Janice M. Reichert, PhD, Executive Director, The Antibody Society, and Editor-in-Chief, mAbs

08:35 TriTAC: A Tri-Specific T Cell Engaging Platform for the Treatment of Solid Tumors

Bryan D. Lemon, PhD, Senior Director, Protein Science, Harpoon Therapeutics, Inc.

T cell engagers are protein therapeutics that tether T cells to surface antigens on tumor cells, leading to activation of those T cells and destruction of the tumor. The TriTAC (tri-specific T cell activating construct) technology is designed to optimize the therapeutic window by addressing half-life and stability limitations of pioneering T cell engagers (e.g., bispecific T bell engagers, or BiTEs). HPN424 first entered the clinic in 2018 and is under development for the treatment of metastatic castration-resistant prostate cancer.

09:05 The Making of a Biparatopic Molecule

Garces_FernandoFernando Garces, PhD, Senior Scientist, Biologics Optimization, Amgen

Multispecifc molecules show great promise as a vehicle to boost pharmacokinetic properties such as therapeutic efficacy and controlled toxicity. Biparatopic antibodies, bispecific molecules that recognize two unique and non-overlapping epitopes on the same antigen/target, have been shown to facilitate receptor internalization, promote superior antagonistic effect, among others. Here, we describe the making of a biparatopic molecule that recognizes two distinct domains on our target receptor. Using a structural-guided approach we have designed, generated and demonstrated that these novel bispecific formats can efficiently bind to both epitopes on the same molecule whereas cross-binding is restricted by the insertion of selected linkers.

09:35 RNAntibody® – A Potent mRNA Technology for Antibody Therapies

Lutz_JohannesJohannes Lutz, PhD, Senior Scientist, CureVac

The delivery of genetic information has emerged as a promising alternative to overcome drawbacks associated with the use of recombinant proteins in protein therapies. Recently, we demonstrated that a single injection of antibody-encoding mRNA rapidly leads to high neutralizing antibody titers in animals, sufficient to provide fast protection against lethal rabies infection or botulinum intoxication. Additionally, we demonstrated that our RNAntibody technology is able to generate therapeutically effective amounts of antibodies in two different mouse models for malignant diseases.

10:05 Presentation to be Announced

10:35 Coffee Break in the Exhibit Hall with Poster Viewing

11:15 Targeted Thorium Conjugates (TTCs): A New Modality for the Treatment of Cancer Utilizing Alpha Particle-Based Radiotherapy

Cuthbertson_AlanAlan Cuthbertson, PhD, Head of Thorium R&D, TCR, Bayer AS

This talk will cover many preclinical aspects of cancer therapy using tumor antigen specific antibodies for the delivery of alpha particle radiation to tumors. The core elements of targeted alpha therapy will be explained along with a selection of data from in vitro and in vivo models demonstrating the potential of this versatile platform.

11:45 The Contorsbody, a Format for Agonism: Design, Structure, Function

Georges_GuyGuy Georges, PhD, Expert Scientist, Large Molecule Research, Roche Innovation Center Munich

The contorsbody is a novel Ab format designed to achieve receptor dimerization. A hybrid structure combining X-ray diffraction and cryo electron microscopy data reveals a special architecture for this format. Depending on receptor and epitope, regular IgG antagonist antibody (ligand blocker or dimerization blocker) can be switched into full agonist when transformed into mono-specific bivalent contorsbody. Bispecific contorsbodies can be obtained via the “Knob into Hole” technology so that receptor hetero-dimerization or hetero-clustering can be achieved.

12:15 Preclinical Development of ELN/22, a Novel “Super-Neutralising” Solomer™ Specific for Human TNF-Alpha

Ubah_ObinnaObinna Ubah, PhD, Senior Research Scientist, Biologics Drug Discovery and Protein Engineering, Elasmogen Limited, Aberdeen

TNFα is implicated in a host of chronic autoimmune inflammatory diseases. Therapeutic targeting and subsequent neutralisation of TNFα has demonstrated positive clinical outcomes, however a significant percentage of these patients fail to respond or have their disease satisfactorily controlled with many patients discontinuing treatment due to life-threatening side effects. The ELN/22 drug candidate is an empirically designed novel biologic with a unique mechanism of TNFα neutralisation and delivers a superior efficacy in an in vivo model of polyarthritis. In addition, the ELN/22 has been designed to reduce the risk of ADA and serious side effects development.

12:45 Talk Title to be Announced

Martin Hoffmann, Senior Scientist, Research & Development – Bioanalytics Frankfurt – Bioprocess Analytics, Sanofi-Aventis Deutschland GmbH

ATUM 13:15 Luncheon Presentation I to be Announced



Ablexis Alivamab_stacked 13:45 Luncheon Presentation II to be Announced

 

 

14:15 Session Break

DEVELOPING SUCCESSFUL ANTIBODY PRODUCTS

14:30 Chairperson’s Remarks

Fernando Garces, PhD, Senior Scientist, Biologics Optimization, Amgen


14:35 FEATURED PRESENTATION: Antibodies to Watch in 2020

Reichert_JaniceJanice M. Reichert, PhD, Executive Director, The Antibody Society, and Editor-in-Chief, mAbs

The “Antibodies to Watch” talks and papers focus on antibody therapeutics in late-stage clinical studies, as well as those in regulatory review and recently approved in the United States and European Union. In this presentation, Dr. Reichert will provide an overview of approvals granted to antibody therapeutics in these regions in 2019, and predict which antibody therapeutics may be approved or move to regulatory review in 2020.

15:05 KEYNOTE PRESENTATION: Explorations in Antibody Product Discovery

Janine Schuurman, PhD, Corporate Vice President Research & Innovation, Antibody Research & Technology, Genmab

The basis for successful product discovery is a conceptual product idea which integrates scientific information on the biology of the disease and the target (or targets), and a thorough understanding of antibody format opportunities. Nevertheless, empirical screening of different target and antibody format combinations is critical, and often adaptations to initial assumptions are required before a novel product fulfilling the anticipated product criteria is discovered: the antibody fine specificity, combined with the format chosen, can have a huge and not always predictable impact on the functional characteristics of novel antibody product. Examples will be shared and discussed.

15:35 Refreshment Break in the Exhibit Hall with Poster Viewing

ANTIBODIES AGAINST MEMBRANE PROTEINS

16:15 Pipeline Update on GPCR and Ion Channel Antibodies

Hutchings_CatherineCatherine Hutchings, PhD, Independent Consultant

G protein-coupled receptors (GPCRs) and ion channels represent some of the most important drug target classes across a wide range of therapeutic areas. An update on antibody-based therapeutics in the pipeline will be provided outlining the breadth and diversity of the target landscape, as well as progress in clinical development. This presentation will also include a summary overview of antigen formats that have been successfully combined with different platforms to address the challenges inherently encountered with complex membrane protein targets.

16:45 Strategies to Isolate and Engineer Functional Antibodies against GPCR and Ion Channel Targets

Wilkinson_TrevorTrevor Wilkinson, PhD, Associate Director, Antibody Discovery and Protein Engineering, AstraZeneca

G-protein-coupled receptors (GPCR) and Ion Channels represent challenging target classes for the isolation and optimization of therapeutic antibodies. In this presentation we review the technical challenges inherent in generating target antigens suitable for antibody isolation and strategies to overcome these challenges. Progress in this area will be illustrated by case studies demonstrating how we have applied phage display and immunization strategies to isolate and optimize functional, antagonistic monoclonal antibodies targeting GPCRs and ion channels.

17:15 ibody AD-214: A Novel Therapy for Fibrosis

Foley_MichaelMichael Foley, PhD, CSO, AdAlta Pty Ltd.

AD-214 is a single domain i-body with affinity for CXCR4, a GPCR which is known to be upregulated in a number of cancers and recently has been implicated in fibrosis. We have shown that AD-214 can block the recruitment of fibrocytes into the lungs of mice with bleomycin induced pulmonary fibrosis and that the anti CXCR4 i-bodies have anti-inflammatory and anti-fibrotic effects in several different animal models of fibrosis.

17:45 Networking Reception in the Exhibit Hall with Poster Viewing

18:45 Problem-Solving Breakout Discussions

Topic: Challenges in Developing Antibodies against GPCRs and Ion Channels

Moderators:

Catherine Hutchings, PhD, Consultant

Trevor Wilkinson, PhD, Assoc Director, Antibody Discovery & Protein Engineering, AstraZeneca Biopharmaceuticals Unit

  • What has been an enablement for use as an antigen format?
  • What screening processes and assays have increased success?
  • Are there emerging trends for how to address specific target classes?
  • What else should we be integrating into the discovery flow?

Topic: New Explorations in Antibody Engineering and Design

Moderator: Guy Georges, PhD, Expert Scientist, Large Molecule Research, Roche Innovation Center Munich

  • Engineer the perfect antibody suited for becoming a therapeutic?
  • Discover the ideal format?
  • Design the adequate bi- multi- specific antibody?

 

 

19:45 End of Day

THURSDAY 21 NOVEMBER

08:00 Registration and Morning Coffee

DEEP SEQUENCING AND HIGH THROUGHPUT SCREENING APPROACHES FOR ANTIBODY DISCOVERY & OPTIMISATION

08:30 Chairperson’s Remarks

Jonny Finlay, PhD, CEO, UltraHuman

08:35 Deep Sequencing of Natural Antibody Repertoires for Antibody Discovery and Optimization and Elucidation of Repertoire Properties

Hotzel_IsidroIsidro Hotzel, PhD, Senior Scientist, Antibody Engineering, Genentech

Hybridoma and B cell cloning remain the main technologies for antibody discovery based on mining of natural immune repertoires. Deep sequencing technologies are now used to enhance repertoire sampling of these technologies for rapid identification of optimized antibody leads and de novo discovery. The application of deep sequencing to repertoire mining coupled to high throughput characterization of antibody panels also provides a broader view of how natural immune repertoires are structured.

09:05 Commonality Despite Exceptional Diversity in the Baseline Human Antibody Repertoire

briney_bryanBryan Briney, PhD, Assistant Professor, Immunology & Microbiology, Scripps Research Institute


09:35 A Comprehensive Screening Platform to Identify the Next Generation Targeted Cancer Immunotherapy Targets

Urlinger_StefanieStefanie Urlinger, PhD, Vice President, Antibody Development, iOmx Therapeutics AG

We have developed a systematic, high-throughput genetic screening approach that enables the identification and comprehensive validation of novel immune checkpoint targets in human cancer cells. Inhibition of these targets by a broad range of methods, such as CRISPR/Cas9 mediated gene knockouts, tool compounds or monoclonal antibodies, results in immune activation and enhanced T cell-mediated tumor cell killing in diverse tumor models. To date, we have validated various novel immune checkpoint targets that drive unique and previously undescribed immune evasion biologies – paving the way for innovative future cancer immunotherapies.

10:05 Presentation to be Announced

10:35 Coffee Break in the Exhibit Hall with Poster Viewing

NOVEL ENGINEERING APPROACHES FOR ANTIBODY DEVELOPMENT

11:15 Antibody Polyspecificity as a Critical Development Risk in Therapeutic Antibody Development

Finlay_JonnyJonny Finlay, PhD, CEO, UltraHuman

UltraHuman Eight has shown for the first time that antibody polyspecificity can be a direct cause of unpredictable side effects in the clinic. Novel insights into identification of off-target binding events and their remediation to create ideal, low-risk clinical leads will be presented.

11:45 Advancing Therapeutic Protein Development Using a Novel O-Glycan-Based Conjugation Approach

Papworth_MonikaMonika Papworth, PhD, Senior Scientist, Antibody Discovery and Protein Engineering, AstraZeneca

Our technology represents an amalgamation of genome editing, cell line metabolic engineering and the application of a novel peptide-based tag to efficiently generate site-specific, homogeneously-labelled recombinant secretory proteins containing modified O-glycans. We demonstrate the facile addition of genetically-encoded O-glycosylation motifs and the robust incorporation of functionalised O-glycans to recombinant proteins using a UDP-galactose-4-eperimase (GALE) knockout, serum-free, cell expression system. This technology represents an elegant, controllable approach to generate bespoke, consistently labelled recombinant proteins ‘On Demand’.

Charles-River 12:15 Luncheon Presentation I: Computational Immuno-Engineering Therapeutics Against Hard Targets: Cracking into GPCR Antagonists & Blood-Brain Barrier

Sarah Ives, Principal Scientist & Director, Contract Research, Distributed Bio

Immune checkpoint inhibitors make attractive yet challenging targets for antibody discovery, where a therapeutic-ready monoclonal antibody can take years to be discovered and subsequently engineered. Here we describe a computational antibody library design that was optimized for both sequence diversity and engineering fitness through the analysis of thousands of human antibody repertoires. The technology enables routine discovery against previously challenging targets including GPCRs, pMHC complexes, and rare epitopes.

SGI-DNA_no-tagline12:45 Luncheon Presentation II: Synthetic Biology Capabilities of the BioXp™ 3200 System

John Gill, Senior Director of Research & Development, Research & Development, SGI-DNA Inc.

The BioXp™ 3200 is a benchtop platform that enables researchers to rapidly synthesize high-quality DNA Libraries, Tiles and Clones in an automated fashion by simply uploading sequence files to a customer portal. The platform dramatically improves workflow productivity for a variety of applications such as protein production, antibody library generation and cell engineering giving more control over genomic workflows. This talk will focus on some of our recent developments for new capabilities on the BioXp.

13:15 Dessert Break in the Exhibit Hall with Poster Viewing

14:00 End of Engineering Antibodies

17:00 Dinner Short Course Registration*

17:3020:30 Dinner Short Courses


Recommended Short Course*

SC6: Selection, Screening and Engineering for Affinity Reagents - LEARN MORE

*Separate registration required.

* The program is subject to change without notice, due to unforeseen reason.

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Update History
2019/10/17
Agenda,Sponsor updated
2019/10/03
Event Information updated
2019/09/09
Sponsor updated
2019/08/23
Sponsor updated
2019/08/09
Sponsor updated
2019/08/06
Agenda updated
2019/06/27
Agenda,Sponsor updated